3/10/2023 0 Comments Sap note 561557However, even at passage 8, there were several predominant lineages that differed with respect to the expression of CD34, CD200 and CD271. After a few passages, virtually all ASCs expressed CD29, CD166 and CD201, in addition to canonical markers CD73, CD90, and CD105. We confirmed that at an early stage, ASC displayed a high heterogeneity with a plethora of subpopulations, which by culturing became more homogeneous. Using multichromatic flow cytometry, ASCs from three different donors each in passages 1, 2, 4, 6, and 8 were analyzed for their co-expression of markers associated with mesenchymal stem cells, wound healing, immune regulation, ASC markers, and differentiation capacity, respectively. In this study, we evaluate the dynamic changes of 15 different surface molecules during culture. However, most of these studies have provided a cross-sectional description of ASCs in either earlier or later passages. The surface marker expression of ASCs has previously been characterized in multiple studies. In order to develop standardized clinical protocols, it is paramount to have a full characterization of the stem cell preparations. Adipose-derived stromal/stem cells (ASCs) are currently being considered for clinical use for a number of indications.
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